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1. Determination of how factors affect the growth rate of E.coli K12.
Time (minutes) Control (Absorbance) Glucose (Absorbance) Lactose
(Absorbance) Glu + Lac (Absorbance)
0 0.09 0.109 0.088 0.088
30 0.112 0.129 0.111 0.111
60 0.145 0.179 0.145 0.159
90 0.143 0.223 0.152 0.201
120 0.142 0.303 0.182 0.269
180 0.147 0.526 0.322 0.449
210 0.151 0.671 0.416 0.488
240 0.159 0.756 0.544 0.601
270 0.165 0.747 0.698 0.749
300 0.171 0.751 0.857 0.864
330 0.177 0.773 0.986 0.937
360 0.174 0.797 1.062 0.961
O/N 0.17 0.801 1.132 0.989
2. Test for β-Galactosidase Activity
Time
(Minutes) Control (Absorbance) Glucose (Absorbance) Lactose
(Absorbance) Glu + Lac (Absorbance)
30 0.344 0.272 0.306 0.347
0.317 0.347 0.351 0.475
0.474 0.333 0.379 0.364
180 0.264 0.719 0.472 2.48
0.288 0.762 0.499 2.48
0.238 0.785 0.463 2.43
330 0.271 2.52 0.707 2.54
0.285 2.59 0.706 2.57
0.318 2.54 0.717 2.59
3. Gram Stain
Control 30 minutes Control 270 Minutes
Glucose 30 minutes Glucose 270 Minutes
Lactose 30 minutes Lactose 270 Minutes
Lactose and Glucose 30 minutes Lactose and Glucose 270 Minutes
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